Figure 3

BiFC of the interaction between LeuC and IPMDHs using the pDOE–mVenus210 system. Nicotiana benthamiana leaves were agroinfiltrated at an optical density of 0.4 and imaged at Day 3. Scale bars = 100 µm. The chlorophyll autofluorescence was detected with the TRITC filter cube (excitation filter: 527.5–565, barrier filter: 577.7–632.5) (top panel), the mVENUS fluorescence was detected using the FITC filter cube (excitation filter: 465–495, barrier filter: 515–155) (middle panel), and the merged images of TRITC and FITC at the bottom panel. (A) NmVen210–X:CVen210 empty vector showing background signal; (B) LeuC:NmVen210–X:CVen210 vector showing background signal; (C) Infiltration buffer control showing background signal; (D) LeuC:NmVen210 interacting with IPMDH1:CVen210; (E) LeuC:NmVen210 interacting with IPMDH2:CVen210; (F) Weak interaction between LeuC:NmVen210 and IPMDH3:CVen210.