Figure 3
Plot of the calcium-probe (GCaMP6s-CAAX) intensity within the footprint of an MCF7 cell during laser damage and repair (a). Intensity is normalized to the maximum intensity reached. The peak maximum at time \(t=t_c\) is indicated (red dot) and a linear fit (blue line) to the intensity decrease during repair. The pump time \(t_p\) is defined as the inverse of the slope of the linear fit. Examples of 10 random intensity curves (probe: GCaMP6s-CAAX) with associated peak maxima and linear fits to the decay region (b). Curves in (b) are offset vertically for clarity. The distribution of measured closure times \(t_c\) and the mean values for the GCaMP6s-CAAX (N = 16) and the GCaMP6s (N = 22) probes (c). The distribution of the pump times \(t_p\) and the mean values for the GCaMP6s-CAAX and the GCaMP6s probes (d). Uncertainties in (c,d) are ± one standard deviation.
