Figure 4
Analysis of the radial distribution of fluorescence intensities from the point of UV-laser damage. Example of a sequence of radial intensity distributions for the probe GCaMP6s-CAAX (a). The mean intensity distribution (solid black) is the time average of the radial intensities. The expectation value of the radius, using the mean intensity distribution, is E(R) and is a measure of the characteristic penetration depth of calcium into the cell after damage. The corresponding standard deviation of the mean radial distribution is \(\sigma \)(R). The scatter and mean value of E(R) for a number of cells labeled with the probes GCaMP6s-CAAX (N = 16) and GCaMP6s (N = 22), respectively (b). The corresponding scatter and mean value of the standard deviation \(\sigma \)(R) for the same cells (C). Uncertainties in (b,c) are ± one standard deviation.
