Figure 5

Output from model of the intracellular calcium distribution. Spatial distribution of the calcium concentration (unit = M) at selected time points before and after hole closure at time \(t_c\) = 6 s (a–f). Diffusion coefficient \(D=2.7\cdot 10^{-11} \, {\mathrm{m}}^2/\mathrm{s}\), hole width \(w_h=1 \, \upmu {\mathrm{m}}\), initial extracellular calcium conc. \(c_o=2\) mM, initial intracellular calcium conc. \(c_i=100\) nM, pump rate \(K=5 \cdot 10^{-7} \, {\mathrm{m}}/\mathrm{s}\). Time dependence of the hole flux \(J_x\) as determined from the solution to the 2-box model in figure 1b (g). Mean concentration inside simulation box normalized to the peak maximum (h). Radial distribution of the calcium concentration relative to the membrane hole at selected time points (i).