Figure 2

Interactions of FUS with Importins. (A) FUS constructs used in (B–E). (B) Pull-down binding assay showing interactions between MBP-FUS, MBP-FUS(1–500), MBP-FUS(R495X), MBP-FUS PY-NLS or Impα with GST-Impβ or GST-Kapβ2 immobilized on beads. Control experiments and unbound proteins in the pull-down experiments are shown in Supplementary Fig. 1A. (C) Pull-down binding assay of MBP-FUS(1–500) with immobilized GST-Kapβ2 in the presence of MBP-FUS PYNLS. (D) Pull-down binding assay to probe interactions between MBP-FUS(1–500) and GST-Kapβ2, -Impα or -Impα/β. (E) Pull-down binding assay of MBP-FUS(1–500) with immobilized GST-Impβ in the presence of MBP-IBB. Proteins in (B–E) are visualized by Coomassie-stained SDS-PAGE.