Table 1 ITC Measurements of Kap2 binding to FUS fragments.

From: Mechanism of karyopherin-β2 binding and nuclear import of ALS variants FUS(P525L) and FUS(R495X)

FUS protein

Residues

KD [2σ] a

FUSPY-NLS

475–526

31 [17-50] nM

FUSPY-NLS (P525L)

475–526

180 [130–240] nM

FUS(1–500)

1–500

160 [120–210] nM

FUS RGG2-ZnF-RGG3

371–500

170 [120–240] nM

FUS RGG2-ZnF

371–452

4 [2–11] μM

FUS RGG3

453–500

6 [3–10] μM

FUS LC-RGG1

1–370

35 [27–46] μM

FUS RRM

278–385

No Binding

FUS ZnF

415–460

No Binding

  1. aThe 95% confidence interval range for KD determined by error-surface projection in the global analysis of the duplicates or triplicates of each experimental set.
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