Table 1 Summary of HDX-MS experimental parameters53.

From: Defining the epitope of a blood–brain barrier crossing single domain antibody specific for the type 1 insulin-like growth factor receptor

Data set

Control

IGF-1

VHH-IR5

HDX reaction details

PBS, pD = 7.0, 25 °C

HDX time course (min)

1, 3, 10, 60

# of Peptides found across all samples

133

Sequence coverage

47%

Average peptide length/redundancy

11.7/1.7

Replicates (biological or technical)

3 (technical)

Repeatability (average standard deviation, %)

1.6

1.5

1.4

Significant differences in HDX

Two-state student T-Test performed at each time point (> 2 SD, p value 0.05)

  1. *All HDX-MS experiments were carried out with recombinant and SEC-purified eIGF1R (Fig. S3).
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