Table 2 Polypeptide and protein constructs used in NMR and related binding studies.

From: Defining the epitope of a blood–brain barrier crossing single domain antibody specific for the type 1 insulin-like growth factor receptor

  1. *(Ubi) denotes the ubiquitin moiety, which has an amino-acid sequence of MQIFVKTLTG-KTITLEVEPS-DTIENVKAKI-QDKEGIPPDQ-QRLIFAGKQL- EDGRTLSDYN-IQKESTLHLV-LRLRGG. Ubi-ID is, therefore, a 157-residue fusion protein and Ubi-s-ID, a 142-residue fusion protein. For Ubi-ID, Ubi-s-ID and Pep5, upper-case letters designate the amino-acid sequence of the uncleaved single-chain human IGF1R precursor1. In lower-case letters are the linker sequence, sgsgsg (for Ubi-ID and Ubi-s-ID), and the c-Myc tag ssgseqkliseed (in VHH-IR5) and a His6 tag added for protein expression and purification. All expressed proteins contain an artificial Met residue at the N-terminus added by the E. Coli host during protein expression.
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