Table 1 Centrifugation and rotor information for MV and exosome isolation.
Centrifugation step | Rotor type | Rotor name | Rotor properties | Centrifugation condition | |||||
|---|---|---|---|---|---|---|---|---|---|
Rmin (mm) | Rmax (mm) | Angle (°) | Lsed (mm) | RCFavg (g) | RPM | Time (min) | |||
1st step | SW | A-4-62 (Eppendorf) | 51 | 178 | – | 127 | 300 | 1500 | 10 |
FA | F-34-6-38 (Eppendorf) | 38.2 | 107 | 34 | 68.8 | 300 | 1900 | 10 | |
2nd step | FA | JA-25.50 (Beckman) | 38.5 | 108 | 34 | 69.5 | 10,000 | 11,000 | 30 |
3rd step | SW | SW 41 Ti (Beckman) | 67.4 | 153.1 | – | 85.7 | 110,000 | 29,800 | 90 |
