Figure 10
3D visulization of residues that substantially contribute to interactions between colicins E5, D, E3 and their cognate immunity proteins. (A) Residues in Im5, ImD, and Im3 that substantially contribute to interactions with their cognate partners. The three interfaces correspond to immunity protein interactions with the cytotoxic domains of the cognate colicins, similar to the compelxes analyzed in Fig. 4. (B) Residues in Im3 that substantially contribute to interactions with the translocation domain (T-domain) of Col-E3. This domain was truncated in the other structures analyzed here. (C) Residues in colicins E5, D and E3 that substantially contribute to interactions with their cognate partners. Only the cytotoxic RNase domains are shown. (D) Residues in the T-domain of Col-E3 that substantially contribute to interactions with Im3. Immunity proteins and colicins/pyocins are shown as ribbons colored light blue and salmon, respectively. The results were visualized on the following structures: Col-E5–Im5 (2FHZ), Col-D–ImD (1V74), Col-E–Im3 (2B5U). All contributing residues are shown as spheres, colored as in Fig. 4.
