Figure 5
Similar electrostatic contributions of colicin/pyocin residues to interactions with the divergent Im α1–α2 motif. Colicin residues that substantially contribute electrostatically to interactions with the Im α1–α2 motif are shown as sticks, colored according to their energy contributions, as in Fig. 3. Colicin residues that contribute similarly to interactions across multiple members are marked with diamonds, colored as follows (with the corresponding E7 position numbering as in Fig. 3B): dark blue (E7#493), light blue (E7#496), teal (E7#520 or E7#540, see also Supplementary Fig. S5), purple (E7#523), red (E7#525), and green (E7#526). Colicin residues that contribute to interactions with the cognate immunity protein only in one complex are marked with black triangles. The colicin/pyocin α2 helices are colored orange, the α4 helices are colored cyan, and the α4–β2 loops are colored green.
