Figure 9
Residues in engineered colicin–immunity protein compexes that are predicted to contribute to inter-molecular interactions in the Im7-like family and E7-like family. (A,B) Residue-level sequence maps summarizing our structure-based energy calculations for the complexes of engineered immunity proteins (A) with their engineered colicins/pyocins (B). Residues that contributed substantially to interactions are colored as in Fig. 3. Mutations that were introduced are marked in bold. Immunity protein and colicin/pyocin positions are numbered according to Im7 (Im7#) and Col-E7 (E7#) and, together with the Im7/E7 secondary structure elements, are shown above and below the alignment.
