Figure 1

A display of cucumber lines fruits morphology and agarose gel resolvability of lnDel markers. Dense spiny cucumber (A), female parent (B) and male parent (C) of Lvmei No.l hybrid variety, Lvmei No.l hybrid variety (D), f ruit cucumber (E), White-green and black spiny cucumber (F), White green cucumber (G), White Green sparsely spin y cucumber (H), Yellow Green sparsely spiny cucumber (I). Agarose gel electrophoresis monograph for poly morphism analysis (J–Q); hybrid seeds purity detection (R–Y) and validation of lnDel markers for 48 cucumber breeding lines (Z–AD); polymorphism analysis using lnDell69 (J), lnDel79 (K); lnDel115 (L); lnDel117 (M), lnDel124 (N); lnDel170 (O); and In Del114 (P–Q); respectively; Hybrid seeds purity testing of the elite cucumber hybrid va riety L vmei No.l using InDel79 (R); InDel170 (S), InDel114 (T–U), InDel124 (V), lnDel232 (W), InDel269 (X), and InDel48 (Y); respectively; Experimental validation of 48 cucumber breeding lines using In Del161 (Z); lnDel174 (AB), lnDel232 (AC), and lnDel269 (AD), X1 and X2 are the parents of elite cucumber hybrid variety Lvmei No.l while C1/C2, C3/C4 and C5/C6 are 3 couples of other three cucumber commercial hybrids while M1/M2, M3/M4 and M5/M6 constitute 3 couples for three commercial melon hybrids; F1 represent individuals of Lvmei No.l; Allele 1, Allele2 and Allele3 are three different allele generated from the 48 lines using InDel269; l = Dl, 2 = D2, 3 = D3, 4 = D4, 5 = D5, 6 = D6, 7 = D7, 8 = D8, 9  =  D9, 10 = D10, 11 = D11, 12 = D12, 13 = D13, 14 = D14, 15 = D15, 16 = D16, 17 = D17, 18 = D18, 19  = D19, 20  = D20, 21 = D21, 22 = D22, 23 = D23, 24 =  B1, 25 =  B2, 26 =  B3, 27 =  B4, 28 =  B5, 29 =  B6, 30 =  B7, 31 =  B8, 32 =  B9, 33 = X1, 34 = X2, 35 = X3, 36 = X4, 37 = X5, 38 = X6, 39 = X7, 40 = X8, 41 = X9, 42 = X10, 43 = X11, 44 = X12, 45 = X13, 46 = X14, 47 = X15, 48 = X16; M represents DL 2000 bp DNA Marker. The uncropped full-length raw gel pictures are included in Supplementary Figure S1.