Figure 3
Results of the open field test, social preference test, and following behavior test. (A) Total distance of movements (left panel), total time spent staying in the center area (middle panel), and total time spent immobile (right panel) in an open field test. There were no significant differences in the results among the groups. (B) Durations and difference between durations of staying in the hand area and the bottle area in a social preference test. The times spent staying in the hand area in the S3-6, S7-10, and S3-10 groups were significantly longer than the time in the N3-10 group. In addition, the time spent staying in the hand area in the S3-10 group was significantly longer than the times in the S3-6, and S7-10 groups. Preference scores [(time spent staying in the hand area) – (time spent staying in the bottle area)] in the S3-6, S7-10, and S3-10 groups were significantly higher than the score in the N3-10 group. (C) Durations of following behavior and number of 50-kHz calls during the following behavior test. Durations of the following behavior (left panel) were significantly longer in the S3-6 and S3-10 groups than in the N3-10 group. In addition, duration of the following behavior was significantly longer in the S3-10 group than in the S7-10 group. The number of 50-kHz calls (right panel) was significantly larger in the S3-10 group than in the N3-10 group. †, P < 0.01; *, P < 0.05. One-way ANOVA followed by post-hoc Holm’s test (A and C, left) and repeated measures two-way ANOVA (group × area) followed by post-hoc Holm’s test (B) were performed. The Kruskal–Wallis test followed by post-hoc Holm’s test was also performed (C, right). Error bars denote standard error of the mean.
