Figure 4 | Scientific Reports

Figure 4

From: Post-weaning stroking stimuli induce affiliative behavior toward humans and influence brain activity in female rats

Figure 4

Numbers of ultrasonic vocalizations. (A) Number of 50-kHz calls. (B) Number of 22-kHz calls. The numbers of 50-kHz calls were significantly larger in the stroking condition than in the basal and holding conditions in the S3-6, S7-10, and S3-10 groups. In addition, in the S3-6, S7-10, and S3-10 groups, the numbers of 50-kHz calls were significantly larger in the holding condition than in the basal condition. In a group comparison, the numbers of 50-kHz calls in the stroking condition in the S3-6 and S3-10 groups were significantly larger than the number in the N3-10 group. The number of 50-kHz calls in the stroking condition in the S3-10 group was significantly larger than that in the S7-10 group. The numbers of 22-kHz calls were not significantly different in the basal, holding, and stroking conditions. (C–F) Numbers of FM and non-FM calls in the N3-10 (C), S3-6 (D), S7-10 (E), and S3-10 (F) groups. In the N3-10, S3-6, and S7-10 groups, the numbers of calls in the stroking condition were significantly larger than those in the basal or holding condition. The number of calls in the holding condition was significantly larger than that in the basal condition. In the S3-10 group, the number of FM calls in the stroking condition was significantly larger than those in the basal and holding conditions. In addition, the number of FM calls was significantly larger than the number of non-FM calls in the stroking condition. The number of non-FM calls in the holding condition was significantly larger than that in the basal condition. †, P < 0.01; *, P < 0.05. a vs. b, P < 0.01; a vs. c, P < 0.01; b vs. c, P < 0.01; d vs. e, P < 0.05; d vs. f, P < 0.05; e vs. f, P < 0.05; g vs. h, P < 0.01; g vs. i, P < 0.01; h vs. i, P < 0.01. A comparison was conducted by repeated measures two-way ANOVA (group × condition) or (syllable pattern × condition) followed by post-hoc Holm’s test. Error bars denote standard error of the mean.

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