Figure 4

Validation of 14 novel miRNAs identified in Ref. Serum. (a) Correlation in expression levels of predicted mature miRNAs measured by qPCR and miRNA-Seq in Ref. Serum. Two miRNAs were not detected above the LOD (data points not shown). r Spearman’s correlation coefficient. (b) Intracellular expression levels of 13 novel miRNAs measured by qPCR across six human cell lines (left y-axis) and read count measured by miRNA-Seq in Ref. Serum (right y-axis). One miRNA was not detected above LOD (data points not shown). (c) Extracellular expression levels of nine novel miRNAs measured by qPCR across six human cell lines (left y-axis) and read count measured by miRNA-Seq in Ref. Serum (right y-axis). Five miRNAs were not detected above LOD (data points not shown). (d) Ratio of extracellular to intracellular expression.