Figure 2

Fluorescence microscopy investigation of cellular components. (A) Representative fluorescence microscopy images of Prochlorococus cells showing the distributions of chlorophylls (green), DNA stained with DAPI (blue), and immuno-stained RubisCO (red). The three columns in the right are merged images of the three. (B) Frequency distribution of component sizes in fluorescence images of the three components. The red and black bars show the length frequencies for the long and short axes, respectively, when each image was roughly approximated as an ellipse circle. (C) Frequency distribution of distances between the centers of chlorophyll and DNA images (red bars) and those between chlorophyll and RubisCO images (black), as illustrated in the inset. (D) Frequency distribution of the distances between the centers of DNA and RubisCO images (red) and those between the center of RubisCO image and envelop of chlorophyll image (black). (E) Schematic illustration of the average distribution of chlorophyll, DNA, and RubisCO in Prochlorococus cells based on panels (B–D).