Figure 3 | Scientific Reports

Figure 3

From: Retinoic acid regulates erythropoietin production cooperatively with hypoxia-inducible factors in human iPSC-derived erythropoietin-producing cells

Figure 3

Chromatin structural changes near the EPO promoter in hiPSC-EPO cells. (A) Effects of hypoxic signals and ATRA treatment on chromatin formation in hiPSC-EPO cells evaluated by Assay for Transposase-Accessible Chromatin Sequencing (ATAC-seq). The ATAC-seq signals were visualized around the EPO TSS under normoxic (21% oxygen) or hypoxic conditions (5% oxygen) with DMSO treatment or under hypoxic condition with ATRA treatment. The y-axes of the ATAC-seq signals represent fragments per million mapped reads (fpm). Red lines indicate peak call regions. (B) qRT-PCR analysis of the mRNA expressions of the genes shown in Table 1. Each value was normalized to the samples treated with DMSO under normoxic conditions. The data from three independent experiments (n = 3) are represented as the means ± SEM. *p < 0.05 by one-way ANOVA with Bonferroni’s test.

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