Figure 2
From: Arginine glycosylation enhances methylglyoxal detoxification
SseK glycosylates multiple MGO detoxification enzymes. (A) Western blot analysis of indicated proteins implicated in MGO detoxification for in vivo Arg-glycosylation in S. enterica. (B) In vivo Arg-glycosylation of GloA, GloB, GloC, and YajL as a function of their co-expression with SseK1, SseK2, and/or SseK3. Original blots are shown in Supplemental Figure S1. (C) In vitro Arg-glycosylation of GloA, GloB, GloC, and YajL after incubation with SseK1 WT or HEN mutant proteins.
