Figure 3
From: Arginine glycosylation enhances methylglyoxal detoxification
In vitro enzyme assays. (A) Purification of as glycosylated or unglycosylated forms of GloA, GloB, GloC, and YajL after their co-expression with SseK1. Western blotting was used to confirm the protein glycosylation state (red, anti-His; green, ant-R-GlcNAc). (B-C) GloA activity assays. (B) Catalysis of S-lactoylglutathione consumption as a function of GloA glycosylation was monitored as a function of time. (C) S-Lactoylglutathione production as a function of GloA glycosylation was monitored as a function of time. (D,E) Glyoxalase II assays. (D) Catalysis of S-lactoylglutathione consumption as a function of GloB-GloC glycosylation. (E) d-lactate production as a function of GloB-GloC glycosylation. (F) YajL repair assay; GAPDH activity was monitored as a function of YajL glycosylation state. Asterisks in panels indicate significantly reaction rates between glycosylated and unglycosylated enzymes.
