Figure 4
From: Arginine glycosylation enhances methylglyoxal detoxification
Glycosylation sites. (A) HCD spectra of the in vivo glycosylated GloB, GloC and YajL peptides 174FALSILPHDSFINEYYRK191, 156GGVGESDFPRGDHTQLIDAIK178, and 143RVVWDARVK151 confirms glycosylation is localized to R190, R160, and R149, respectively. (B) Western blot analysis of in vitro GloB and GloC glycosylation assays. (C) Western blot analysis of in vitro YajL glycosylation assays. (D) Site-directed mutagenesis and Western blot analysis to map the GloA glycosylation site to R9.
