Figure 7
Role of caspase 8 and 9 in apoptosis induction of B16 melanoma cells: This was determined by TUNNEL assay. Cells were treated at different concentrations with APL (Psiadia lithospermifolia) and vehicle or in the presence of inhibitors of caspase 8 and 9. (A–D) represent treatment without any inhibitors of caspases where (A) (vehicle DMSO), (B) (1 µg/ml), (C) (10 µg/ml), (D) (25 µg/ml) of APL. (E–H) represent treatment with APL in presence of caspase 8 inhibitor. (E) (vehicle DMSO), (F) (1 µg/ml), (G) (10 µg/ml), (H) (25 µg/ml) of APL extract. (I–L) represent treatment with APL in presence of caspase 9 inhibitor. (I) (vehicle DMSO), (J) (1 µg/ml), (K) (10 µg/ml), (L) (25 µg/ml) of APL extract. The data presented are representative of three independent experiments. Bar chart (M) shows quantitative data of average of two independent flow cytometry experiments in which the values were expressed relative to no inhibitor. The significance between various groups was tested using one-way ANOVA where NS: Not significant; (*)p < 0.05, (**)p < 0.01.
