Figure 2 | Scientific Reports

Figure 2

From: Development and validation of a multiplex qPCR assay for detection and relative quantification of HPV16 and HPV18 E6 and E7 oncogenes

Figure 2

Sensibility and linearity of HPV16 and HPV18 triplexes. Standard curves were generated by amplification of serial dilutions from 107 to 10–2 of E6, E7 and GAPDH plasmid pool for HPV16 (a–c) and HPV18 (d–f) using multiplex qPCR. Each dilution was run in biological and technical triplicate. R2 represent the coefficient of determination and the PCR efficiency (E) of each target was calculated using the slope of each standard curve with the formula E = (10–1/slope − 1)*100.

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