Figure 5
Determination of physical tissue volume from a convex hull spanned between nuclei reidentified over time. (a) Stable patterns of nuclei were reidentified over all timepoints and nucleus centre coordinates were sampled as fiducial markers throughout all stack dimensions. Two examples of reidentified nuclei are indicated by blue arrows. Maximum intensity Z-projection of 10 optical sections covering 20 µm. Scale bar = 20 µm. (b) Fiducial marker coordinates plotted inside single stack volume. Dot colours specify fiducials that could be reidentified reliably at all three time points. Grid lines spaced in 100 µm. (c) Three-dimensional Delaunay triangulation from fiducials create matching tetrahedra. All tetrahedra create a convex hull (entire volume in blue) whose subtle volume changes indicate shrinkage or expansion of the tissue within.
