Figure 6
Correlation of tissue volume with GMV. Colours of plotted datapoints denote individual mice. (a) Volumes of convex hulls in 2Pii stacks significantly shrink over time. Display of all measured absolute volumes. Repeated measure one-way ANOVA with complete datasets (all timepoints available) of n = 18 positions of 7 mice, F = 29.12, DF = 2, p = < 0.0001. Post-hoc Tukey test for multiple comparisons with adjusted p values: 0 vs. 1 week: p = 0.260; 0 vs. 12 weeks: p < 0.0001; 1 vs. 12 weeks: p < 0.0001. (b) Relative change in 2Pii convex hull volumes compared to baseline (= theoretical mean of 100%) at 1 week and 12 weeks. In addition to Fig. 6A, also datapoints with missing values for the 12 weeks timepoint are included. At 1 week: n = 22 positions of 9 mice, mean ± SD = 98.74 ± 2.55%; Wilxocon signed rank test gives p = 0.046. At 12 weeks n = 19 positions of 7 mice, mean ± SD = 95.16 ± 2.71%, Wilxocon signed rank test gives p < 0.0001; linear mixed effect model for difference between 1 and 12 weeks: p < 0.0001. (c) Changes of GMV in MRI masks of 2Pii stacks. Wilxocon signed rank test at 1 week gives mean ± SD = 99.87 ± 0.24%, p < 0.001, n = 45 positions of 9 mice; at 12 weeks: mean ± SD = 99.67% ± 0.71%, p = 0.075, n = 35 positions of 7 mice. Linear mixed effect model for difference 1 week and 12 weeks: p = 0.008. (d) Microscopic volume changes correlate with GMV in MRI masks. Linear mixed effect model with p = 0.014, Estimate = 0.046%, R2 = 0.130, n = 41 (22 positions of 9 mice at 1 week, 18 positions of 7 mice at 12 weeks).
