Figure 7

Correlations of GMV with cell number, nearest neighbour distance and mean nucleus volume. (a,d,g) n = 60 positions from 10 mice in week 0, 45 positions from 9 mice in week 1, 35 positions from 7 mice in week 12. (b,c,e,f,h,i) At 1 week: n = 45 positions from 9 mice; at 12 weeks: n = 35 positions of 7 mice. (a) Number of cells within the whole 2Pii stack as counted by automated detection. Mean ± SD over all 2Pii stacks is given in thousands: baseline = 19.50 ± 5.46; 1 week = 18.70 ± 4.31, 12 weeks = 19.77 ± 4.31. Linear mixed effect model with pairwise contrasting and correction for multiple comparison: week 1 vs. baseline: p = 0.12, week 12 vs. baseline: p = 0.55, week 12 vs. week 1: p = 0.73. (b) Relative change in the number of cells compared to baseline. Mean ± SD over all 2Pii stacks is given in % of baseline: 1 week = 91.26 ± 11.70; One sample t test gives p < 0.001, DF = 44, t = -5.009. 12 weeks = 92.72 ± 13.30; one-sample t test gives t = -3.24, DF = 34, p = 0.003. Linear mixed effect model for difference between 1 and 12 weeks: p = 0.430. (c) Correlation of GMV change and change of cell count. Linear mixed effect model: p = 0.303. (d) Mean nearest neighbour distance. Mean ± SD over all 2Pii stacks in µm: baseline = 12.89 ± 0.67; 1 week = 12.97 ± 0.42, 12 weeks = 12.96 ± 0.39. Linear mixed effect model with pairwise contrasting and correction for multiple comparison: week 1 vs. baseline: p = 0.030, week 12 vs. baseline: p = 0.025, week 12 vs. week 1: p = 0.950. (e) Relative change in nearest neighbour distances compared to baseline. Mean ± SD over all 2Pii stacks is given in % of baseline. 1 week: = 101.27 ± 2.05; one-sample t test gives t = 4.145, DF = 44, p < 0.001. 12 weeks = 101.27 ± 2.39; one-sample t test gives t = 3.156, DF = 34, p = 0.003. Linear mixed effect model for difference between 1 and 12 weeks: p = 0.871. (f) Correlation of GMV change with change of NN distance. Linear mixed effect model: p = 0.411. (g) Mean nuclear volume. Mean ± SD over all 2Pii stacks is given in µm³: baseline = 1054 ± 43; 1 week = 1039 ± 47; 12 weeks = 1060 ± 53. Linear mixed effect model with pairwise contrasting and correction for multiple comparison: week 1 vs. baseline: p = 0.167, week 12 vs. baseline: p = 0.962, week 12 vs. week 1: p = 0.155. (h) Relative change in mean nucleus volume compared to baseline. Mean ± SD over all 2Pii stacks is given in % of baseline. 1 week = 98.54 ± 3.34; one-sample t test gives t = -2.93, DF = 44, p = 0.005. 12 weeks = 99.67 ± 4.59; one-sample t test gives t = -0.43, DF = 34, p = 0.670. Linear mixed effect model for difference between 1 and 12 weeks: p = 0.080. (i) Correlation of GMV and mean nucleus volume. Linear mixed effect model: p = 0.002, Estimate = − 0.039%, R² = 0.091.