Figure 3 | Scientific Reports

Figure 3

From: GDF11 alleviates secondary brain injury after intracerebral hemorrhage via attenuating mitochondrial dynamic abnormality and dysfunction

Figure 3

GDF11 attenuated EH-induced apoptosis and ROS accumulation in SH-SY5Y cells. (A,B) Quantification of apoptosis and cell released LDH in culture exposed to 0.1–10 μM Hb for 24 h. (Values shown as mean ± SD. *p < 0.05 and **p < 0.01 versus control group, n = 3). The cell cultures for EH treatment of the following experiments were exposed to 5 μM Hb environment for 24 h. (C,D) Bar graph and Flow cytometric quantification of apoptosis percentage presenting as fold increase (n = 7). (E,F) The TUNEL-positive cells presenting as fold increases and representative pictures for TUNEL/DAPI-fluorescence staining (scale bar 200 μm). (G-J) Quantification of immunoreactive bands for Bax (G), and Bcl-2 (H) relative to β-actin, and cleaved Caspase-3 (I) relative to Caspase-3. Immunoreactive bands for Bax, Bcl-2, cleaved Caspase-3, and Caspase-3 in the different group (J). Values shown as mean ± SD. *p < 0.05 and **p < 0.01 versus control group; #p < 0.05 versus EH group and &p < 0.05 versus control group (n = 7).

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