Table 2 QTL analysis of fruit traits in bitter gourd in F_2:3 family lines.

S. No	Trait	QTL*	LG^y	QTL Position (cM)	Flanking markers		Marker closest to peak	Closeness of marker to trait (cM)	R² (%)	LOD	Additive effect^#
S. No	Trait	QTL*	LG^y	QTL Position (cM)	Left marker	Right marker	Marker closest to peak	Closeness of marker to trait (cM)	R² (%)	LOD	Additive effect^#
1	Fruit length (cm)	qFL1	1	28.81	TP_3003	TP_2693	TP_3003	1.51	9.69	2.7	− 1.84
		qFL5	5	77.48	TP_11213	TP_11334	TP_11334	1.82	13.04	3.6	− 2.12
		qFL14	14	55.54	TP_67839	TP_68143	TP_67839	0.04	11.21	3.4	2.02
2	Fruit diameter (cm)	qFD1	1	55.8	TP_1877	TP_1459	TP_1459	0.10	9.63	2.6	− 0.60
		qFD3	3	55.86	TP_8845	TP_8817	TP_8817	0.74	0.10	15.8	0.06
		qFD13	13	90.49	TP_57231	TP_56896	TP_57231	0.49	0.27	9.3	0.10
		qFD15	15	66.54	TP_66813	TP_66819	TP_66813	0.04	6.16	2.5	0.49
		qFD16	16	77.12	TP_74581	TP_74591	TP_74591	4.78	32.65	15.7	− 1.12
		qFD20	20	53	TP_78027	TP_75976	TP_78027	0.50	9.46	2.8	− 0.61
3	Fruit weight (g)	qFW1	1	55.8	TP_1877	TP_1459	TP_1459	0.10	10.81	3.1	− 12.48
		qFW15	15	71.88	TP_67045	TP_67087	TP_67045	2.48	9.17	3.6	11.82
		qFW20	20	55.5	TP_78027	TP_75976	TP_78027	3.00	9.36	2.6	− 11.62
4	Fruit flesh thickness (mm)	qFT14	14	80.12	TP_68612	TP_68958	TP_68958	0.48	0.09	2.6	0.10
5	Number of fruits per plant	qFN5	5	76.98	TP_11213	TP_11334	TP_11334	2.32	9.07	2.5	− 2.66
		qFN14	14	122.25	TP_69841	TP_69846	TP_69841	1.85	8.20	2.6	− 2.51
		qFN20	20	50.47	TP_78598	TP_78027	TP_78027	2.03	13.81	3.6	− 3.27
6	Yield per plant (g)	qYD1	1	55.8	TP_1877	TP_1459	TP_1459	0.10	10.19	3.1	− 223.07
		qYD15	15	71.88	TP_67045	TP_67087	TP_67045	2.48	4.88	2.6	161.47
		qYD20	20	53	TP_78027	TP_75976	TP_78027	0.50	8.21	2.5	− 200.97

*QTL named as qxxxy, with “xxx” being the trait abbreviation, ‘y’ the number of the linkage group. # Additive effect was positive when the “DBGy-201” allele increased the trait score and was negative when the “Pusa Do Mausami” allele increased trait score. The 95% confidence interval of the QTL locations was determined with 2-LOD support interval which was defined by left and right markers.

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