Figure 4 | Scientific Reports

Figure 4

From: Chemosensitizing activity of peptide from Lentinus squarrosulus (Mont.) on cisplatin-induced apoptosis in human lung cancer cells

Figure 4

L. squarrosulus peptide modulates apoptosis and survival pathway in human lung cancer cells. (a) Western blot analysis revealed the reduction of anti-apoptosis proteins (Mcl-1, Bcl-2) in H460 cells cultured with 5 µg/mL of L. squarrosulus peptide from 0.4 M NaCl fraction for 12–24 h. (b) However, the significant upregulation of p53 and pro-apoptosis Bax protein was observed after 3 h of peptide treatment. The downregulation of (c) survival proteins and (e) upstream regulatory molecules, integrins, was also detected in L. squarrosulus peptide treated-cells. (d) The expression level of survival p-FAK, FAK, p-Src, Src, p-Akt and Akt protein was time-dependently decreased which corresponded with (f) the diminution of integrin β1, β3, β5, αV and α5 in H460 cells incubated with 5 µg/mL of 0.4 M NaCl peptide. Values are means of three independent experiments ± SD. *p < 0.05 versus non-treated control cells.

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