Figure 5 | Scientific Reports

Figure 5

From: Chemosensitizing activity of peptide from Lentinus squarrosulus (Mont.) on cisplatin-induced apoptosis in human lung cancer cells

Figure 5

Pretreatment with 0.4 M NaCl peptide obtained from L. squarrosulus significantly sensitizes cisplatin-induced cell death through regulation of apoptosis and integrin-mediated survival pathway in human lung cancer H460 cells. (a, b) The protein levels of Mcl-1 and Bcl-2 were significantly decreased while tumor suppressor p53 and pro-apoptosis Bax protein levels were obviously increased in comparison with H460 cells treated with cisplatin alone. (c, d) Related survival proteins (FAK, p-FAK, Src, p-Src, Akt, p-Akt) and (e, f) integrins (α5, αV, β1, β3, β5) were considerably decreased as a result of peptide preincubation in cisplatin-treated cells compared to the cells treated with cisplatin alone. Values are means of three independent experiments ± SD. *p < 0.05 versus non-treated control cells, #p < 0.05 versus the cells only treated with cisplatin.

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