Figure 5

pMEA recordings of P2X7-KO mice demonstrate dysfunction of OFF-RGCs, without rescue, and additional dysfunction of ON-RGC following acute IOP elevation. (A) Spontaneous activity was increased in ON and ON–OFF RGCs and (B) light-elicited responses were reduced in ON- and OFF-RGCs following elevated IOP (red bars). (C) The latency to peak response was increased in OFF-RGCs following elevated IOP. Numbers indicate the number of cells recorded from n = 6 animals. (D–F) The spontaneous activity and light-elicited responses were averaged per retina and are presented per retina for ON- (D), OFF- (E) and ON–OFF (F) RGCs. Insets show the spontaneous rate of cells from sham and elevated IOP eyes. Both ON- and OFF-RGCs from the P2X7-KO mouse showed reduced light responsiveness following acute IOP elevation*p < 0.05, **p < 0.01, ****p < 0.0001; Mann–Whitney test (sham vs IOP).