Figure 6
P2X7-Rs are highly expressed in ON-RGCs of Thy1-YFP-H mice. Thy1-YFP-H RGCs (green) are projections of the original confocal Z-stacks, while the P2X7-R puncta contacting the dendrites (red) are digitally rendered as spots (presented as 3 times larger than the true colocalised puncta for ease of viewing). (A–C) Thy1-YFP-H labelled ON-RGC (green) showing dense labelling for P2X7-R puncta (red) shown in (A) orthogonal view, (B) from above and (C) magnified inset from (B). (D) A single plane of raw data from the ON-cell (green) with P2X7-R staining (red). (E–G) Thy1-YFP-H labelled OFF-RGC (green) showing sparser labelling for P2X7-R puncta (red) than ON-RGCs. The OFF-RGC is shown in (E) orthogonal view, (F) from above and (G) magnified inset from F. (H–L) Thy1-YFP-H labelled ON–OFF RGC (green) in the orthogonal view (H) displaying dendritic fields stratifying in ON and OFF sublaminae. Labelling for the P2X7-R puncta (red) in the ON sublamina (I) from above and (J) magnified inset from (I). Labelling for the P2X7-R puncta (red) in the OFF sublamina (K) from above and (L) magnified inset from K. (M) Quantification of P2X7-R puncta in the ON and OFF lamina of the IPL for ON-, OFF- and ON–OFF RGCs. Numbers indicate number of cells from 4 animals. *p < 0.01; one-way ANOVA, Tukey’s multiple comparisons test. Scale bar is 10 µm.
