Figure 10

Screenshots of sublingual microcirculation obtained by incident dark-field imaging and examples of space–time diagrams generated from the microvascular units indicated in red in the images. (1.a) Example of sublingual microcirculation. In this image, one capillary-post-capillary venule unit is shown by the redlined outlines of the vessel walls. Dimensions of the field of view of the sublingual microcirculation screenshot is 1.55 mm × 1.16 mm. (1.b) Shows the generated space–time diagram of the capillary-post capillary venule. The space–time diagram shows several straight white bands, indicating non-rolling leukocytes flowing through the capillary and post capillary venule without rolling or contact with the vessel wall. The white band is a straight line, corresponding with the straight yellow, control line. (2.a) Example of sublingual microcirculation. In this image, one capillary-post capillary venule unit is shown by the redlined outlines of the vessel walls. Dimensions of the field of view of this sublingual microcirculation screenshot is 1.55 mm × 1.16 mm. (2.b) Shows the generated space–time diagram of the capillary-post capillary venule. The space–time diagram shows several white bands that are not straight, indicating rolling leukocytes flowing through the capillary and getting into contact with the endothelial wall in the post capillary venule part. The white band is slanted, and the straight yellow line drawn on it, does not fit the white band of this rolling leukocyte. The yellow arrow shows the start of the slanting of the white band, this is also the moment at which the white band is not straight anymore due to significant change in the velocity of the leukocyte by rolling or adherence to the endothelium. S the axis of the space–time diagram for space, Time the axis of the space–time diagram indicating the time.