Scheme 3

In situ regeneration of the acetyltransfer co-factor 5, with acetylthiocholine iodide 16, during the one-pot three enzyme synthesis of the Pse5Ac7Ac biosynthetic intermediate 17.

In situ regeneration of the acetyltransfer co-factor 5, with acetylthiocholine iodide 16, during the one-pot three enzyme synthesis of the Pse5Ac7Ac biosynthetic intermediate 17.