Table 1 Conditions used in the induction of Pse5Ac7Ac biosynthetic enzymes and the resulting quantity of enzyme.

From: Reconstitution and optimisation of the biosynthesis of bacterial sugar pseudaminic acid (Pse5Ac7Ac) enables preparative enzymatic synthesis of CMP-Pse5Ac7Ac

 

Molecular weight/kDa

IPTG concn/mM

Induction temp/°C

Induction time/h

Enzyme quantity/mg L−1

PseB

37.4

0.1

37

4

14

PseC

42.3

0.1

16

4

9

PseH

18.7

0.1

16

20

17

PseG

31.3

0.5

37

4

11

PseI

38.6

0.5

37

20

18

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