Figure 5 | Scientific Reports

Figure 5

From: Mechanical stretch induces Ca2+ influx and extracellular release of PGE2 through Piezo1 activation in trabecular meshwork cells

Figure 5

The impact of Piezo1 knockdown on the release of arachidonic acid and PGE2 following mechanical stretch stimulation in human trabecular meshwork cells. In human trabecular meshwork cells transfected with control or Piezo1 siRNAs, Cell supernatants were collected 10, 30, and 60 min after stretch stimulation. The levels of arachidonic acid (A), PGE2 (B) in the cell supernatant were measured. Piezo1 knockdown suppressed the release of arachidonic acid (A) and PGE2 (B). Data are expressed as mean ± SE (n = 3 samples of lipid mediators extracted from cell supernatant in 3 chambers). *p < 0.05. **p < 0.01, two-tailed Student’s t-test.

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