Figure 6

Direct activation of Piezo1 by Yoda1 induces the release of arachidonic acid and PGE2 in human trabecular meshwork cells. Human trabecular meshwork cells were incubated for the indicated time with or without Yoda1, and lipid mediators in the medium were analyzed. Yoda1 triggered the release of arachidonic acid (A) and PGE2 (B), but not PGD2 (C) in human trabecular meshwork cells. Data are expressed as the means ± SE (n = 3 samples of lipid mediators extracted from cell supernatant in 3 chambers). **p < 0.01, two-tailed Student’s t-test.