Figure 4 | Scientific Reports

Figure 4

From: Thermal reaction norms of key metabolic enzymes reflect divergent physiological and behavioral adaptations of closely related amphipod species

Figure 4

Temperature-dependent expression of RNA transcripts of key metabolic enzymes. Transcripts of citrate synthase (CS) and 3-hydroxyacyl-CoA dehydrogenase (HADH) were analyzed in whole animal extracts of E. verrucosus, E. cyaneus, and G. lacustris upon exposure of the animals to gradual temperature increase (0.8 °C d−1). Samples of all three species were taken at 6 °C, 12.4 °C, 18.8 °C, and 23.6 °C; besides, samples of E. verrucosus were taken at 9.2 °C, 15.6 °C, and 22 °C. Expression data are expressed as log2fold change calculated against the same endogenous controls (actin and gapdh) for all three species. Temperature-treated groups are shown with filled circles, whereas the time control groups (kept at 6 °C) are represented by open circles. Asterisks indicate data points outside the range of biological insignificance (± 0.5 log2fold change; dotted horizontal lines), which are significantly different from the 6 °C treatment group (ANOVA, p < 0.05). Data are presented as means ± s.e.m. (n = 5–7).

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