Table 1 Genes and primer sequences for real-time PCR. The primer pairs were derived from the genes of interest from the respective species and used after validation (see material and methods) for RT-PCR for the respective organism: E. verrucosus (Ev), E. cyaneus (Ec), and G. lacustris (Gl).
Organism | Gene ID | Annotation (Swissprot) | Accession No | Forward-Primer | Backward primer | Used for: |
|---|---|---|---|---|---|---|
E. verrucosus | CISY | Citrate synthase | KX831868 | CACCTCCCTGATGACAACCTTT | GCCGGGCACCACAGTATAA | Ev, Ec |
GGAGTGCCAACTTCACCAACA | GCCTCATGAGCTCGGTAAACTG | Gl | ||||
COX4 | Cytochrome c oxidase subunit IV | KX831869 | GAAAAGGAAAAGGGTGACTGGAA | AGATGCTCGGTATAAGGCCTTCT | Ev, Ec, Gl | |
COX2 | Cytochrome c oxidase subunit II | YP_008964129 | GTAGCCCTACCAATTAACACTCAAATT | CCGCCCATGAGTGAATAACG | Ev | |
GLUD1 | Glutamate dehydrogenase 1, mitochondrial | KX831872 | GTGGTGTCACCGTTTCCTACTTT | GGTGAGCCTGCCGTAAGAGA | Ev, Ec, Gl | |
ECHA | Trifunctional enzyme subunit alpha, mitochondrial, includes: 3-hydroxyacyl-CoA dehydrogenase | KX831870 | AAGTGGTGATTGTGGTGAAGGA | AACATGGGTGCCAGGATACG | Ev, Ec | |
LDH-A | Lactate dehydrogenase A chain, muscle isoform | KX831873 | TCGGCCTGTCCGTAGCA | GCAACGTCTCTGGTTCTTCATG | Ev, Ec | |
PKM | Pyruvate kinase, muscle isoform | KX831875 | CGCCATCATCGTCATTACCA | AACGAGGCCGGTACTTGGA | Ev | |
E. cyaneus | ACT | Actin | JN860427 | CCGCCGAGCGAGAAATC | GGGCCACGTAGCAAAGCTT | Ec, Ev, Gl |
G3P | Glyceraldehyde-3-phosphate dehydrogenase | KF293381 | GGCAAAGGTCCACTTCAACAA | GGCGGAGGGAGCAGAGAT | Ec, Ev, Gl | |
PKM | Pyruvate kinase, muscle isoform | KX844831 | CTGCGGTGGAGGCTTCTTT | GTGGTCGTGATGACGATGATG | Ec | |
G. lacustris | ECHA | Trifunctional enzyme subunit alpha, mitochondrial, includes: 3-hydroxyacyl-CoA dehydrogenase | KX831871 | GGCCTATGGAGCAGAGTTTGA | TTCCAGATTTGGCGTAGTCTTG | Gl |
LDH-A | Lactate dehydrogenase A chain, muscle isoform | KX831874 | GCCTGCGCGACCTCAA | CGTTGTACTTGTCAGGGTCATTG | Gl | |
PKM | Pyruvate kinase, muscle isoform | KX831876 | CTGCCGCAACATAGATTCCA | TCACCGCGAGCGATCAT | Gl |
