Table 4 Nitrate, TSNA, and alkaloid contents of F1 hybrids between 35S:S523D-NR lines and late flowering cultivars in 2017.

NO3N (ppm)

1274 ± 160.5^A

1467 ± 201.3^A

1596 ± 226.9^A

23 ± 2.2^C

28 ± 5.5^C

25 ± 2.6^C

56 ± 18.3^B

22 ± 2.3^C

44 ± 4.4^B

NNN (ng/g)

421 ± 99^A

176 ± 32^B

133 ± 12^B

89 ± 6^C

89 ± 4^C

87 ± 5^C

87 ± 4^C

84 ± 5^C

92 ± 6^C

NNK (ng/g)

98 ± 12.1^A

82 ± 13.5^A

96 ± 12.1^A

39 ± 3.5^B

41 ± 3.1^B

33 ± 2.3^B

39 ± 3.8^B

33 ± 3.6^B

44 ± 5.0^B

NAT (ng/g)

566 ± 181^A

302 ± 93^B

163 ± 29^B

110 ± 12^B

118 ± 10^B

95 ± 7^B

90 ± 6^B

99 ± 13^B

96 ± 8^B

NAB (ng/g)

25 ± 4.36^A

31 ± 8.10^A

24 ± 5.89^A

15 ± 3.19^AB

12 ± 1.24^AB

13 ± 2.73^B

11 ± 1.20^AB

9 ± 0.93^B

12 ± 1.69^AB

Total TSNA (ng/g)

1110 ± 291^A

591 ± 142^B

374 ± 63^B

252 ± 18^C

259 ± 15^C

227 ± 12^C

227 ± 11^C

225 ± 20^C

244 ± 19^C

Nicotine (%)

3.4 ± 0.31^AB

2.1 ± 0.30^CD

1.5 ± 0.19^D

4.1 ± 0.29^AB

4.3 ± 0.24^A

3.8 ± 0.27^AB

3.0 ± 0.30^BC

3.5 ± 0.30^AB

3.1 ± 0.31^ABC

Nornicotine (%)

0.066 ± 0.0062^AB

0.021 ± 0.0023^C

0.017 ± 0.0012^C

0.078 ± 0.0077^AB

0.085 ± 0.0063^A

0.069 ± 0.0072^AB

0.055 ± 0.0068^B

0.058 ± 0.0062^B

0.056 ± 0.0066^B

Anabasine (%)

0.012 ± 0.0012^BCD

0.008 ± 0.0011^DE

0.005 ± 0.0006^E

0.017 ± 0.0016^AB

0.019 ± 0.0014^A

0.015 ± 0.0012^ABC

0.011 ± 0.0014^CD

0.014 ± 0.0013^BC

0.013 ± 0.0016^BCD

Anatabine (%)

0.101 ± 0.013^C

0.052 ± 0.009^D

0.036 ± 0.004^D

0.157 ± 0.014^AB

0.181 ± 0.014^A

0.131 ± 0.011^BC

0.097 ± 0.015^C

0.115 ± 0.012^BC

0.103 ± 0.013^C

TA (%)

3.6 ± 0.33^AB

2.2 ± 0.31^CD

1.5 ± 0.19^D

4.3 ± 0.31^AB

4.6 ± 0.26^A

4.0 ± 0.28^AB

3.2 ± 0.32^BC

3.7 ± 0.31^AB

3.3 ± 0.33^ABC