Table 5 Nitrate, TSNA, and alkaloid contents of F1 hybrids between 35S:S523D-NR lines and late flowering cultivars in 2018.

NO3N (ppm)

2366.5 ± 238.2^B

3207.9 ± 212.6^A

2182.8 ± 143.8^B

27.3 ± 3.7^D

23.4 ± 1.8^D

48.4 ± 12.0^C

33.7 ± 4.3^CD

51.4 ± 8.0^C

45.3 ± 5.4^C

NNN (ng/g)

317.2 ± 63.9^A

125.2 ± 8.3^B

98.3 ± 6.2^BC

95.8 ± 8.1^BC

91.6 ± 6.7^BC

90.2 ± 8.7^C

66.5 ± 3.6^D

93.6 ± 5.2^BC

71.4 ± 8.9^D

NNK (ng/g)

89.4 ± 9.5^A

65.0 ± 6.2^B

58.7 ± 3.0^B

40.0 ± 2.9^C

36.5 ± 3.1^C

38.9 ± 3.6^C

29.6 ± 1.9^C

34.5 ± 2.9^C

30.5 ± 3.3^C

NAT (ng/g)

273.0 ± 54.6^A

189.2 ± 28.4^A

146.0 ± 12.0^AB

107.9 ± 10.7^BC

107.0 ± 7.7^BC

95.8 ± 6.6^CD

75.2 ± 4.1^D

103.8 ± 6.3^BC

82.0 ± 14.1^D

Total TSNA (ng/g)

680 ± 124.7^A

379 ± 40.4^AB

303 ± 19.6^BC

244 ± 20.8^C

235 ± 17.0^C

225 ± 17.7^C

171 ± 8.6^D

232 ± 13.5^C

184 ± 25.8^D

Nicotine (%)

3.0 ± 0.32^BC

2.5 ± 0.23^C

2.1 ± 0.17^C

3.9 ± 0.31^AB

4.4 ± 0.23^A

3.3 ± 0.37^ABC

2.5 ± 0.31^C

3.7 ± 0.20^AB

2.6 ± 0.35^C

Nornicotine (%)

0.051 ± 0.0054^A

0.015 ± 0.0016^C

0.012 ± 0.0012^C

0.056 ± 0.0070^A

0.064 ± 0.0052^A

0.047 ± 0.0054^AB

0.032 ± 0.0040^B

0.050 ± 0.0035^A

0.033 ± 0.0055^B

Anabasine (%)

0.0084 ± 0.00109^BCD

0.0063 ± 0.00100^CD

0.0052 ± 0.00055^D

0.0122 ± 0.00219^AB

0.0151 ± 0.00127^A

0.0112 ± 0.00143^ABC

0.0072 ± 0.00132^BCD

0.0127 ± 0.00094^AB

0.0076 ± 0.00161^BCD

Anatabine (%)

0.057 ± 0.0086^CDE

0.038 ± 0.0065^E

0.036 ± 0.0033^E

0.112 ± 0.0141^AB

0.120 ± 0.0111^A

0.078 ± 0.0107^BCD

0.053 ± 0.0102^DE

0.089 ± 0.0078^ABC

0.056 ± 0.0137^CDE

TA (%)

3.1 ± 0.33^BCD

2.6 ± 0.24^CD

2.2 ± 0.18^D

4.1 ± 0.33^AB

4.6 ± 0.24^A

3.4 ± 0.38^ABC

2.6 ± 0.33^CD

3.9 ± 0.21^AB

2.7 ± 0.37^CD