Figure 5 | Scientific Reports

Figure 5

From: Click-correlative light and electron microscopy (click-AT-CLEM) for imaging and tracking azido-functionalized sphingolipids in bacteria

Figure 5

Quantification of azido-functionalized sphingolipid levels in membrane fractions of N. meningitidis by mass spectrometry. Characterization of membrane fractions (1–12) of N. meningitidis after treatment of bacteria with either ω-N3-sphingosine (a) or ω-N3-C6-ceramide (b). Each fraction was tested for LDH activity per µg protein (blue line), as inner membrane marker and OpcA abundance by dot blot (below X-axis) as outer membrane marker. Bars represent the amount of azido-functionalized sphingosine or ceramide, as determined by HPLC–MS/MS, normalized to the amount of C16 sphingomyelin (SM) found in the samples. LDH data show the mean ± SD of a representative experiment performed in duplicate.

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