Figure 2

Schematic of persistence concept and analysis. (A) Conceptual framework for persistence, which considers protein turnover and RNA fold change together to gain a deeper understanding of the functional impact of changes in gene expression by estimating changes in protein expression. High persistence genes (orange) are thought to have a greater functional impact because they are produced more (high RNA expression) and remain in the cell longer (low protein turnover). Low persistence genes (blue) are thought to have a lesser functional impact because they are not produced as much (low RNA expression) and are cleared from the cell faster (high protein turnover). In this way, the persistence formula considers protein turnover and RNA fold change together and generates persistence scores provide deeper functional insights into RNA changes by acting as a proxy for protein changes. (B) Outline of code used to produce persistence scores and identify genes of high or low persistence. See Supplemental Code 1 for more details.