Figure 2 | Scientific Reports

Figure 2

From: The ADP-binding kinase region of Ire1 directly contributes to its responsiveness to endoplasmic reticulum stress

Figure 2

Effect of the Y225H mutation on the UPR inducibility of Ire1 mutants. KMY1015 cells (ire1Δ) bearing the UPRE-lacZ reporter plasmid, pCZY1, were transformed with the IRE1 plasmid, pRS315-IRE1-HA (wild-type: WT), or with that carrying the indicated mutations (or with the empty vector, pRS315), and were grown at 30 °C in SD medium. For ER stress induction, we added tunicamycin (2 µg/mL) into the cultures, which were further cultured for 4 h. The cultures were then subjected to the measurement of cellular β-galactosidase activity, and the resulting values are normalized against that of wild-type (WT) IRE1 cells, which is set at 1.0.

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