Figure 3 | Scientific Reports

Figure 3

From: The ADP-binding kinase region of Ire1 directly contributes to its responsiveness to endoplasmic reticulum stress

Figure 3

Effect of the Y225H mutation on the physical interaction between the Ire1 luminal domain and unfolded proteins. After denaturation in guanidine HCl solution, luciferase (25 μM) or citrate synthase (50 μM) were 40- (for luciferase) or 100-fold (for citrate synthase) diluted in assay buffer containing MBP-cLD (wild-type: WT) or its mutant versions (1 µM each) or in buffer only, and further incubated at 25 °C for 5 min. The turbidity of the sample mixtures was then measured, normalized against that of the “Buffer only” sample, which is set at 1.0 and presented as “Aggregation”.

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