Table 2 Bovine primers used in qRT-PCR.

From: Epidermal growth factor alleviates the negative impact of urea on frozen-thawed bovine sperm, but the subsequent developmental competence is compromised

Gene

 

Sequence of nucleotide (5′–3′)*

Tm (℃)

Product size, bp

Accession No

BAX

F

AGCGAGTGTTCTGAAGCG

61

 

AC_000175.1

R

CCCAGTTGAAGTTGCCG

182

BCL-2

F

CCTTCTTTGAGTTCGGAG

60

 

AC_000181.1

R

CCTTCAGAGACAGCCAG

121

ACTB

F

CCATCGGCAATGAGCGGT

60

 

AC_000182.1

R

CGTGTTGGCGTAGAGGTC

146

  1. Tm melting temperature, F forward, R reverse.