Figure 1 | Scientific Reports

Figure 1

From: SPARC coordinates extracellular matrix remodeling and efficient recruitment to and migration of antigen-specific T cells in the brain following infection

Figure 1

Parenchymal SHG signal is distinct from neurons and brain vasculature. (a) All imaging, multiphoton and confocal, focused on the frontal cortex of the mouse brain, highlighted in blue. (b) Thy1.1-YFP reporter mice were infected with Pru-OVA for 14 days before fresh (unfixed) brains were imaged using multiphoton microscopy to visualize fluorescent neurons (green) and SHG signal (blue). (c) Similarly, C57Bl/6 mice infected with Pru-OVA for 14 days were injected with the fluorescent dye SRB (red) i.v. 30 min prior to multiphoton imaging to distinguish the parenchymal SHG signal (blue) from that generated by collagen rich blood vessels. (d) SHG signal (blue) in naïve mice and following traumatic brain injury. Green fluorescence (*) indicates vascular leakage of FITC Dextran at injury site. For all panels, 30 μm z-stacks were collected in areas of the frontal cortex from fresh tissue explants. Images are representative of at least 2 individual experiments where n = 3 mice.

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