Figure 2 | Scientific Reports

Figure 2

From: Rapid in situ quantification of the strobilurin resistance mutation G143A in the wheat pathogen Blumeria graminis f. sp. tritici

Figure 2

Sensitivity and specificity of the dPCR and ASqPCR assays targeting the cytb G143A. (a) Scatter plots for Blumeria graminis f. sp tritici (Bgt) G143A dPCR assay using known ratios of genomic DNA for the strobilurin resistant Bgt sample Geelong 1 (A143 allele) and sensitive Bgt sample Goomalling (G143 allele). Scatter plots were prepared with Quantstudio 3D AnalysisSuite. Wells with the A143 allele are represented by blue signals (FAM), G143 alleles are represented by red signals (VIC), detection of both alleles are represented by green signals, wells without any alleles (passive reference) are represented by yellow signals (ROX) and outliers represented by grey signals. (i) 100% Goomalling gDNA (G143 allele only); A143 frequency = 0.04%. (ii) 0.33% Geelong1 gDNA; A143 frequency = 0.23%. (iii) 3.39% Geelong 1 gDNA; A143 frequency = 3.10%. (iv) 6.90% Geelong 1 gDNA; A143 frequency = 7.87%. (b) Correlation of threshold cycle in ASqPCR and amount of DNA of either G143 (R2 = 0.9998) or A143 (R2 = 0.9999) alleles, respectively.

Back to article page