Table 2 PCR, Digital PCR and qPCR primers and probes used in this study.

From: Rapid in situ quantification of the strobilurin resistance mutation G143A in the wheat pathogen Blumeria graminis f. sp. tritici

Primer namea

Sequence (5′–3′)

Product size (bp)

Anneal temperature (°C)

Purpose

Source

BgCytB_G143A_F

CTGCATTCCTGGGTTATGTATTGC

86

See below

dPCR/qPCR primers

This study

BgCytB_G143A_R

TGGTATAGCGCTCATTAGGTTAGTGA

BgCytB_G143A_dP1

FAM-CGGTTGCAGCCCAGTG-NFQ

-

58

dPCR probes

This study

BgCytB_G143A_dP2

VIC-ACGGTTGCACCCCAGTG-NFQ

BgCytB_G143A_mP1

FAM-CGGTTGCAGCCCAGTG-BHQ1

-

67

qPCR probes

This study

BgCytB_G143A_mP2

HEX-ACGGTTGCACCCCAGTG-BHQ1

WM-Cb-f

GCGGGATGTTAATAACGGATGAT

465

55

Sequencing primers

Bäumler et al. (2003)

WM-Cb-R

GGAGCCATAGGTAATCTATCG

  1. aThe probes dP1 and mP1 target the cytb A143 allele whereas probes dP2 and mP2 target the G143 wild-type allele. Italics—fluorophore/quencher; bold—single nucleotide polymorphism; underlined—locked nucleic acid.
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