Table 4 Sensitivity of the G143A dPCR and ASqPCR assays on a mixture of total DNA of leaves infected with mutant (A143) and wild type (G143) Blumeria graminis f. sp. tritici.

From: Rapid in situ quantification of the strobilurin resistance mutation G143A in the wheat pathogen Blumeria graminis f. sp. tritici

DNA samplea

dPCR

ASqPCR

% A143

CI % A143b

% A143

CI % A143

100% Goo

0.04

1.19E-2–0.16

0

–

0.07% Gee 1

0.30

0.18–0.51

0

–

0.17% Gee 1

0.10

4.41E-2–0.25

0

–

0.33% Gee 1

0.23

0.13–0.42

0

–

0.67% Gee 1

0.57

0.37–0.88

0

–

1.68% Gee 1

2.01

1.63–2.48

1.48

1.32–1.64

3.39% Gee 1

3.10

2.61–3.68

4.06

3.82–4.30

6.90% Gee 1

7.87

6.98–8.87

8.00

7.22–8.77

18.18% Gee 1

21.72

19.98–23.59

20.24

19.96–20.51

40% Gee 1

38.45

36.00–41.04

NAc

–

66.67% Gee 1

65.15

61.41–69.05

68.40

67.90–68.89

100% Gee 1

99.67

94.43–105.11

100

–

  1. aGoo Goomalling (G143), Gee1 Geelong 1 (A143). bCI 95% confidence interval. cThis concentration was not tested with ASqPCR.
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